MetaMouse® Publications

General

  1. Dutton, G.  AntiCancer Inc. scientists identify a key governing step in the metastasis of cancer.  Genetic Engineering News 16, 1, January 15, 1996. [PDF]

  2. Holzman, D.  Of mice and metastasis: A new for-profit model emerges.  Journal of the National Cancer Institute 88, 396-397, 1996.[PDF] 

  3. Riordan, T.  A technique is said to ease attachment of tumors to mice, making them ‘little cancer patients.’  New York Times, “Patents” Column, March 4, 1996. [PDF]

  4. Hoffman, R.M.  Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Investigational New Drugs 17, 343-359, 1999.[PDF] 

  5. Dove, A.  Illuminating cancer models.  Nature Biotechnology 18, 261, 2000. [PDF]

  6. McCann, J.  Jellyfish protein gives new glow to tumor imaging.  Journal of the National Cancer Institute 93, 976-977, 2001[PDF].  

  7. Garber, K.   Realistic rodents?  Debate grows over new mouse models of cancer.  Journal of the National Cancer Institute 98, 1176-1178, 2006.

  8. Garber, K.   From human to mouse and back:  “Tumorgraft” models surge in popularity.  Journal of the National Cancer Institute 101, 6-8, 2009.

Bladder

  1. Fu, X., Theodorescu, D., Kerbel, R.S., and Hoffman, R.M.  Extensive multi-organ metastasis following orthotopic onplantation of histologically-intact human bladder carcinoma tissue in nude mice.  Int. J. Cancer 49, 938-939, 1991.[PDF] 

  2. Fu, X., and Hoffman, R.M.  Human RT-4 bladder carcinoma is highly metastatic in nude mice and comparable to ras-H-transformed RT-4 when orthotopically onplanted as histologically intact tissue.  Int. J. Cancer 51, 989-991, 1992.[PDF]   

  3. Chang, S-G., Kim, J.I., Jung, J-C., Rho, Y-S., Lee, K-T., An, Z., Wang, X., and Hoffman, R.M.  Antimetastatic activity of the new platinum analog {Pt(cis-dach)(DPPE)×2NO3} in a metastatic model of human bladder cancer.  Anticancer Res. 17, 3239-3242, 1997.[PDF] 

  4. Zhou, J-H., Rosser, C.J., Tanaka, M., Yang, M., Baranov, E., Hoffman, R.M., Benedict, W.F. Visualizing superficial human bladder cancer cell growth in vivo by green fluorescent protein expression.  Cancer Gene Therapy 9, 681-686, 2002. [PDF]

Bone cancer and metastasis

  1. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression. Cancer Research 58, 4217-4221, 1998
  2. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M. A fluorescent orthotopic bone metastasis model of human prostate cancer. Cancer Research 59, 781-786, 1999
  3. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Research 5, 3549-3559, 1999
  4. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000
  5. Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005
  6. Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005.
  7. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model. Clinical Cancer Research 12, 2602-2606, 2006
  8. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M. Systemic targeting of primary bone tumor and lung metastasis of high-grade osteosarcoma in nude mice with a tumor-selective strain of Salmonella typhymurium. Cell Cycle 8, 870-875, 2009.
  9. Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., and Hoffman, R.M. Cancer metastasis directly eradicated by targeted therapy with a modified Salmonella typhymurium. J. Cell. Biochem. 106, 992-998, 2009.
  10. Tome, Y., Tsuchiya, H., Hayashi, K., Yamauchi, K., Sugimoto, N., Kanaya, F., Tomita, K., and Hoffman, R.M. In vivo gene transfer between interacting human osteosarcoma cell lines is associated with acquisition of enhanced metastatic potential. J. Cell. Biochem. 108, 362-367, 2009. DOI: 10.1002/jcb.22259.
  11. Ongkeko, W.M., Burton, D., Kiang, A., Abhold, E., Kuo, S.Z., Rahimy, E., Yang, M., Hoffman, R.M., Wang-Rodriguez, J., Deftos, L.J. Parathyroid hormone related-protein promotes epithelial-to-mesenchymal transition in prostate cancer. PLoS One 9, e85803, 2014.

Brain cancer and metastasis 

  1. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M.  A fluorescent orthotopic bone metastasis model of human prostate cancer.  Cancer Research 59, 781-786, 1999.

  2. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Research 5, 3549-3559, 1999.

  3.  Hoffman, R.M.  Green fluorescent protein imaging of tumor cells in mice.  Lab Animal 31, 34-41, 2002.

  4.  Hoffman, R.M., and Yang, M.  Whole-body imaging with fluorescent proteins.  Nature Protocols 1, 1429-1438, 2006.

  5. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M.  A color-coded orthotopic nude-mouse treatment model of brain-metastatic paralyzing spinal cord cancer that induces angiogenesis and neurogenesis.  Cell Proliferation 42, 75-82, 2009.

  6. Hoffman, R.M.  Comment re:  Preclinical model of spontaneous melanoma metastasis.  Cancer Research 69, 719, 2009.

  7. Folkins, C., Shaked, Y., Man, S., Tang, T., Lee, C.R., Zhu, Z., Hoffman, R.M., and Kerbel, R.S.  Glioma tumor stem-like cells promote tumor angiogenesis and vasculogenesis via vascular endothelial growth factor and stromal-derived factor 1.  Cancer Research69, 7243-7251, 2009.

  8. Kimura, H., Zhang, L., Zhao, M., Hayashi, K., Tsuchiya, H., Tomita, K., Bouvet, M., Wessels, J., and Hoffman, R.M.  Targeted therapy of spinal cord glioma with a genetically-modified Salmonella typhimurium.  Cell Proliferation 43, 41-48, 2010.

  9.  Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R.  Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo.  PLoS One 5, e9712, 2010.  DOI: 10.1371/journal.pone.0009712.

  10. Soda, Y., Marumoto, T., Friedmann-Morvinski, D., Soda, M., Liu, F., Michiue, H., Pastorino, S., Yang, M., Hoffman, R.M., Kesari, S., Verma, I.M.  Transdifferentiation of glioblastoma cells into vascular endothelial cells.  Proc. Natl. Acad. Sci. USA 108, 4274-4280, 2011. Commentary:  Hormigo, A., Ding, B-S., and Rafii, S.  A target for antiangiogenic therapy: Vascular endothelium derived from glioblastoma.  Proc. Natl. Acad. Sci. USA 108, 4271-4272, 2011.

  11. Momiyama, M., Suetsugu, A., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M.  Subcellular real-time imaging of the efficacy of temozolomide on cancer cells in the brain of live mice.  Anticancer Res. 33, 103-106, 2013.

  12. Momiyama, M., Suetsugu, A., Kimura, H., Chishima, T., Bouvet, M., Endo, I., and Hoffman, R.M. Dynamic subcellular imaging of cancer cell mitosis in the brain of live mice. Anticancer Res. 33, 1367-1371, 2013.

  13. Momiyama, M., Zhao, M., Kimura, H., Tran, B., Chishima, T., Bouvet, M., Endo, I., and Hoffman R.M. Inhibition and eradication of human glioma with tumor-targeting Salmonella typhimurium in an orthotopic nude-mouse model. Cell Cycle 11, 628-632, 2012.

  14. Momiyama, M., Hiroshima, Y., Suetsugu, A., Tome, Y., Mii, S., Yano, S., Bouvet, M., Chishima, T., Endo, I., and Hoffman, R.M. Enhanced resection of orthotopic red-fluorescent-protein-expressing human glioma by fluorescence-guided surgery in nude mice. Anticancer Res. 33, 107-112, 2013.

  15. Rashidi, B., Moossa, A.R., and Hoffman, R.M. Specific route mapping visualized with GFP of single-file streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J. Cell. Biochem 114, 1738-1743, 2013.

Breast

  1. Fu, X., Le, P., and Hoffman, R.M.  A metastatic orthotopic-transplant nude-mouse model of human patient breast cancer.  Anticancer Res. 13, 901-904, 1993. [PDF]

  2. Kubota, T., Inoue, S., Furukawa, T., Ishibiki, K., Kitajima, M., Kawamura, E., and Hoffman, R.M.  Similarity of serum – Tumor pharmacokinetics of antitumor agents in man and nude mice.  Anticancer Res. 13, 1481-1484, 1993.[PDF]

  3.  Li, X-M., Wang, J-W., An, Z., Yang, M. Baranov, E., Jiang, P., Sun, F-X., Moossa, A.R., and Hoffman, R.M. Optically imageable metastatic model of human breast cancer. Clinical & Experimental Metastasis, 19, 347-350, 2002. [PDF]

  4. John, C.M., Leffler, H., Kahl-Knutsson, B., Svensson, I., Jarvis, G.A. Truncated Galectin-3 Inhibits Tumor Growth and Metastasis in Orthotopic Nude Mouse Model of Human Breast Cancer. Clinical Cancer Res. 9, 3808-3814, 2003 [PDF]

  5. Goodison, S., Kawai, K., Hihara, J., Jiang, P., Yang, M., Urquidi, V., Hoffman, R.M., and Tarin, D. Prolonged dormancy and site-specific growth potential of cancer cells spontaneously disseminated from non-metastatic breast tumors revealed by labeling with green fluorescent protein. Clinical Cancer Res. 9, 3808-3814, 2003. [PDF] 

  6. Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moosa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in portal vein area. Cancer Res. 66, 303-306, 2006.[PDF]

  7.  Drapeau, C., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., and Schaeffer, D.J.  The stem cell mobilizer StemEnhance does not promote tumor growth in an orthotopic model of human breast cancer.  Anticancer Research 29, 443-447, 2009.

  8. Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R.  Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo.  PLoS One 5, e9712, 2010.  DOI: 10.1371/journal.pone.0009712.

  9. Tan, W., Zhang, W., Strasner, A., Grivennikov, S., Cheng, J.Q., Hoffman, R.M., and Karin, M.  Tumour-infiltrating regulatory T cells stimulate mammary cancer metastasis through RANKL-RANK signalling.  Nature 470, 548-553, 2011.

  10. Zhang, Q., Bindokas, V.P., Shen, J., Fan, H., Hoffman, R.M., Xing, H.R.  Time-course imaging of therapeutic functional tumor vascular normalization by anti-angiogenic agents.  Mol. Cancer Ther. 10, 1173-1184, 2011.

  11. Hu, M., Zhao, M., An, C., Yang, M., Li, Q., Zhang, Y., Suetsugu, A., Tome, Y., Yano, S., Fu, Y., Hoffman, R.M., and Hu, K.  Real-time imaging of apoptosis induction of human breast cancer cells by the traditional Chinese medicinal herb Tubeimu.  Anticancer Res. 32, 2509-2514, 2012.

  12. Zhang, Y., Tome, Y., Suetsugu, A., Zhang, L., Zhang, N., Hoffman, R.M., and Zhao M.  Determination of the optimal route of administration ofSalmonella typhimurium A1-R to target breast cancer in nude mice.  Anticancer Research 32, 2501-2508, 2012.

  13. Suetsugu, A., Honma, K., Saji, S., Moriwaki, H., Ochiya, T., and Hoffman, R.M. Imaging exosome transfer from breast cancer cells to stroma at metastatic sites in orthotopic nude mouse models. Adv. Drug Deliv. Rev. 65, 383-390, 2013.

  14. Hoffman, R.M. Stromal-cell and cancer-cell exosomes leading the metastatic exodus for the promised niche. Breast Cancer Res. 15, 310-313, 2013.

  15. Wu, J., Ma, R., Cao, H., Wang, Z., Jing, C., Sun, Y., Zhang, Y., Yang, Z., Hoffman, R.M., and Tang, J. Intraoperative imaging of metastatic lymph nodes using a fluorophore-conjugated antibody in a HER2/neu-expressing orthotopic breast cancer mouse model. Anticancer Research 33, 419-424, 2013.

  16. Ke, C-C., Liu, R-S., Suetsugu, A., Kimura, H., Ho, J.H., Lee, O.K., and Hoffman, R.M. In vivo fluorescence imaging reveals the promotion of mammary tumorigenesis by mesenchymal stromal cells. PLoS One 8, e69658, 2013.

Colon

  1. Fu, X., Besterman, J.M., Monosov, A., and Hoffman, R.M.  Models of human metastatic colon cancer in nude mice orthotopically constructed by using histologically intact patient specimens.  Proc. Natl. Acad. Sci. USA 88, 9345-9349, 1991.[PDF]

  2. Fu, X., Herrera, H., Kubota, T., and Hoffman, R.M.  Extensive liver metastasis from human colon cancer in nude and SCID mice after orthotopic onplantation of histologically-intact human colon carcinoma tissue.  Anticancer Res. 12, 1395-1398, 1992.[PDF] 

  3. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T.H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  Immunochemotherapy prevents human colon cancer metastasis after orthotopic onplantation of histologically-intact tumor tissue in nude mice.  Anticancer Res. 13, 287-291, 1993. [PDF] 

  4. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  Early resection of primary orthotopically-growing human colon tumor in nude mouse prevents liver metastasis: further evidence for patient-like hematogenous metastatic route.  Anticancer Res. 13, 293-297, 1993. [PDF]

  5. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Nishibori, H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., and Kitajima, M.  A metastatic model of human colon cancer constructed using cecal implantation of cancer tissue in nude mice.  Jpn. J. Surg. 23, 420-423, 1993. [PDF].

  6. Wang, X., Fu, X., Brown, P.D., Crimmin, M.J., and Hoffman, R.M.  Matrix metalloproteinase inhibitor BB-94 (Batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice.  Cancer Res. 54, 4726-4728, 1994.. [PDF] 

  7. Togo, S., Shimada, H., Kubota, T., Moossa, A.R., Hoffman, R.M.  “Seed” to “soil” is a return trip in metastasis. Anticancer Res. 15, 791-794, 1995.[PDF]

  8. Togo, S., Shimada, H., Kubota, T., Moossa, A.R., Hoffman, R.M.  Host organ specifically determines cancer progression.  Cancer Res. 55, 681-684, 1995.[PDF] 

  9. Kuo, T-H., Kubota, T., Watanbe, M., Furukawa, T., Teramoto, T., Ishibiki, K., Kitajimi, M., Moossa, A.R., Penman, S., Hoffman, R.M.  Liver colonization competence governs colon cancer metastasis.  Proc. Natl. Acad. Sci. USA 92, 12085-12089, 1995.[PDF]

  10. Togo, S., Wang, X., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Cancer seed and soil can be highly selective: Human-patient colon tumor lung metastasis grows in nude mouse lung but not colon or subcutis.  Anticancer Res. 15, 795-798, 1995.[PDF]

  11. Leff, D.N.  MetaMouse models colon cancer metastasis with clinical potential.  BioWorld Today 7, 1, January 8, 1996. [PDF] 

  12. Murray, G., Duncan, M., O’Neil, P., Melvin, W., Fothergill, J.  Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer.  Nature Medicine 2, 461-462, 1996. [PDF]

  13. An, Z., Wang, X., Willmott, N., Chander, S.K., Tickle, S., Docherty, A.J., Mountain, A., Millican, A.T., Morphy, R., Porter, J.R., Epemolu, R.O., Kubota, T., Moossa, A.R., and Hoffman, R.M.  Conversion of highly malignant colon cancer from an aggressive to a controlled disease by oral administration of a metalloproteinase inhibitor.  Clinical & Experimental Metastasis 15, 184-195, 1997. [PDF]

  14. Sun, F-X., Sasson, A.R., Jiang, P., An, Z., Gamagami, R., Li, L., Moossa, A.R., and Hoffman, R.M.  An ultra-metastatic model of human colon cancer in nude mice. Clinical & Experimental Metastasis 17, 41-48, 1999. [PDF]

  15. Sasson, A.R., Gamagami, R., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M.  Cimetidine: An inhibitor or promoter of tumor growth?  International Journal of Cancer 81, 835-838, 1999. [PDF]

  16. Rho, Y-S., Lee, K-T., Jung, J-C., Yoon, C., An, Z., Hoffman, R.M., and Chang, S-G.  Efficacy of new platinum analog DPPE in an orthotopic nude mouse model of human colon cancer.  Anticancer Res. 19, 157-162, 1999. [PDF]

  17. Rashidi, B., An, Z., Sun, F-X., Sasson, A., Gamagammi, R., Moossa, A.R., Hoffman, R.M.  Minimal liver resection strongly stimulates the growth of human colon cancer in the liver of nude mice.  Clinical & Experimental Metastasis 17, 497-500, 1999. [PDF]

  18. Rashidi, B., Sun, F-X., Jiang, P., An, Z., Gamagami, R., Moossa, A.R., and Hoffman, R.M.  A nude mouse model of massive liver and lymph node metastasis of human colon cancer. Anticancer Res. 20, 715-722, 2000. [PDF] 

  19. Rashidi, B., An, Z., Sun, F-X., Moossa, A.R., and Hoffman, R.M.  Antimetastatic intraoperative chemotherapy of human colon tumors in the livers of nude mice.  Clinical Cancer Res. 6, 2464-2468, 2000.[PDF] 

  20. Rashidi, B., Gamagami, R., Sasson, A., Sun, F-X., Geller, J., Moossa, A.R., and Hoffman, R.M.  An orthotopic mouse model of remetastasis of human colon cancer liver metastasis.  Clinical Cancer Res. 6, 2556-2561, 2000. [PDF] 

  21. Tsuji, K., Yamauchi, K., Yang, M., Jiang, P., Bouvet, M., Endo, H., Kanai, Y., Yamashita, K., Moosa, A.R., and Hoffman, R.M. Dual-color imaging of nuclear-cytoplasmic dynamics, viability, and proliferation of cancer cells in portal vein area. Cancer Res. 66, 303-306, 2006.[PDF]

  22. Lukashev, M., LePage, D., Wilson, C., Bailly, V., Garber, E., Lukashin, A., Ngam-ek, A., Zeng, W., Allaire, N., Perrin, S., Xu, X., Szeliga, K., Wortham, K., Kelly, R., Bottiglio, C., Ding, J., Griffith, L., Heaney, G., Silverio, E., Yang, W., Jarpe, M., Fawell, S., Reff, M., Carmillo, A., Miatkowski, K., Amatucci, J., Crowell, T., Prentice, H., Meier, W., Violette, S.M., Mackay, F., Yang, D., Hoffman, R., and Browning, J.L. Targeting the lymphotoxin-beta receptor with agonist antibodies as a potential cancer therapy. Cancer Research 66, 9617-9624, 2006.[PDF]

  23. Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer. Anticancer Res. 27, 713-718, 2007.[PDF]

  24. Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M. Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma. J. Surg. Res. 140, 165-170, 2007. [PDF]

  25. Guo, X-N., Rajput, A., Rose, R., Hauser, J., Beko, A., Kuropatwinski, K., LeVea, C., Hoffman, R.M., Brattain, M.G., and Wang, J. Mutant PIK3CA-bearing colon cancer cells display increased metastasis in an orthotopic model. Cancer Res. 67, 5851-5858, 2007. [PDF]

  26. Rajput, A., Dominguez San Martin, I., Rose, R., Beko, A., Levea, C., Sharratt, E., Mazurchuk, R., Hoffman, R.M., Brattain, M.G., and Wang, J. Characterization of HCT116 human colon cancer cells in an orthotopic model. J. Surg. Res. 147, 276-281, 2008.

  27. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., Yamashita, S. In vitro/in vivo biorecognition of lectin-immobilized fluorescent nanospheres for human colorectal cancer cells. Journal of Controlled Release 134, 2-10, 2009.

  28. Ma, H., Das, T., Pereira, S., Yang, Z., Zhao, M., Mukerji, P., and Hoffman, R.M. Efficacy of dietary antioxidants combined with a chemotherapeutic agent on human colon cancer progression in a fluorescent orthotopic mouse model. Anticancer Research 29, 2421-2426, 2009.

  29. Sakuma, S., Yano, T., Masaoka, Y., Kataoka, M., Hiwatari, K.I., Tachikawa, H., Shoji, Y., Kimura, R., Ma, H., Yang, Z., Tang, L., Hoffman, R.M., and Yamashtia, S. Detection of early colorectal cancer imaged with peanut agglutinin-immobilized fluorescent nanospheres having surface poly(N-vinylacetamide) chains. Eur. J. Pharma. Biopharma. 74, 451-460, 2010. DOI: 10.1016/j.ejpb.2010.01.001.

  30. Ishihara, Y., Matsunaga, K., Iijima, H., Hasegawa, G., Suzuki, T., Sato, A., Kobayashi, T., Yang, M., and Hoffman, R.M. The combination of 5-FU, leucovorin and CPT-11 (FOLFIRI) prolongs survival through inhibition of metastasis in an orthotopic model of colon cancer. Anticancer Res. 30, 403-408, 2010.

  31. Suetsugu, A., Osawa, Y., Nagaki, M., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M. Imaging the recruitment of cancer-associated fibroblasts by liver-metastatic colon cancer. J. Cell. Biochem. 112, 949-953, 2011.

  32. Kishimoto, H., Aki, R., Urata, Y., Bouvet, M., Momiyama, M., Tanaka, N., Fujiwara, T., Hoffman, R.M. Tumor-selective adenoviral-mediated GFP genetic labeling of human cancer in the live mouse reports future recurrence after resection.  Cell Cycle 10, 2737-2741, 2011.

  33. Sakuma, S., Yamashita, S., Hiwatari, K., Hoffman, R.M., and Pham, W.  Lectin-immobilized fluorescent nanospheres for targeting to colorectal cancer from a physicochemical perspective.  Curr. Drug Discov. Technol. 8, 367-378, 2011.

  34. Momiyama, M., Kumamoto, T., Suetsugu, A., Kishimoto, H., Chishima, T., Tanaka, K., Akiyama, H., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M.  Major liver resection stimulates stromal recruitment and metastasis compared with repeated minor resection.  J. Surg. Res. 178, 280-287, 2012.

  35. Metildi, C.A., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet, M.  Fluorescence-guided surgery of human colon cancer increases complete resection resulting in cures in an orthotopic nude mouse model.  J. Surg. Res. 179, 87-93, 2013.

Esophageal

  1. Gros, S.J., Dohrmann, T., Peldschus, K., Schurr, P.G., Kaifi, J.T., Kalinina, T., Reichelt, U., Mann, O., Strate, T.G., Adam, G., Hoffman, R.M., and Izbicki, J.R.  Complementary use of fluorescence and magnetic resonance imaging of metastatic esophageal cancer in a novel orthotopic mouse model.  International Journal of Cancer 126, 2671-2681, 2010.

  2. Gros, S., Kurschat, N., Dohrmann, T., Reichelt, U., Dancau, A-M., Peldschus, K., Adam, G., Hoffman, R.M., Izbicki, J.R., and Kaifi, J.T.  Effective therapeutic targeting of the overexpressed HER-2 receptor in a highly metastatic orthotopic model of esophageal carcinoma.  Molecular Cancer Therapeutics 9, 2037-2045, 2010.

  3. Gros, S.J., Dohrmann, T., Kaifi, J.T., Bouvet, M., Hoffman, R.M., Izbicki, J.R.  Orthotopic fluorescent peritoneal carcinomatosis model of esophageal cancer.  Anticancer Research 30, 3933-3938, 2010.

  4. Gros., S.J., Kurschat, N., Drenckhan, A., Dohrmann, T., Forberich, E., Effenberger, K., Reichelt, U., Hoffman, R.M., Pantel, K., Kaifi, J.T., and Izbicki, J.R.  Involvement of CXCR-4 chemokine receptor in metastastic HER2-positive esophageal cancer.  PLoS One7(10), e47287, 2012.

Gene Therapy

  1. Miki, K., Xu, M., An, Z., Wang, X., Yang, M., Al-Refaie, W., Sun, X., Baranov, E., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  Survival efficacy of the combination of the methioninase gene and methioninase in a lung cancer orthotopic model.  Cancer Gene Therapy 7, 332-338, 2000. . [PDF]

  2. Miki, K., Al-Refaie, W., Xu, M., Jiang, P., Tan, Y., Bouvet, M., Zhao, M., Gupta, A., Chishima, T., Shimada, H., Makuuchi, M., Moossa, A.R., and Hoffman, R.M.  Methioninase gene therapy of human cancer cells is synergistic with recombinant methioninase treatment.  Cancer Res. 60, 2696-2702, 2000.[PDF]

  3. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000. [PDF]

  4. Djeha, A.H., Thomson, T.A., Leung, H., Searle, P.F., Young, L.S., Kerr, D.J., Harris, P.A., Mountain, A., and Wrighton, C.J.  Combined adenovirus-mediated nitroreductase gene delivery and CB1954 treatment: A well-tolerated therapy for established solid tumors.  Molecular Therapy 3, 233-240, 2001. [PDF] 

  5. Chen, C.T., Lin, J., Li, Q., Phipps, S.S., Jakubczak, J.L., Stewart, D.A., Skripchenko, Y., Forry-Schaudies, S., Wood, J., Schnell, C., and Hallenbeck, P.L.  Antiangiogenic gene therapy for cancer via systemic administration of adenoviral vectors expressing secretable endostatin.  Human Gene Therapy 11, 1983-1996, 2000. [PDF]

  6. Miki, K, Xu, M., Gupta, A., Ba, Y., Tan, Y., Al-Refaie, W., Bouvet, M., Makuuchi, M., Moossa, A.R., and Hoffman, R.M.  Methioninase cancer gene therapy with selenomethionine as suicide prodrug substrate.  Cancer Research 61, 6805-6810, 2001. [PDF] 

  7. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H.  VEGF receptor antisense therapy inhibits angiogenesis and peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy 9, 197-201, 2002. [PDF]

  8. Pirocanac, E.C., Nassirpour, R., Yang, M., Wang, J-W., Nardin, S.R., Gu, J., Fang, B., Moossa, A.R., Hoffman, R.M., and Bouvet, M.  Bax-induction gene therapy of pancreatic cancer.  J. Surg. Res. 106, 346-351, 2002. [PDF]

  9. Hoffman, R.M. Immune reactions in skin and hair follicle gene therapy. Molecular Therapy 7, 294-295, 2003.

  10. Katz, M.H., Spivak, D.E., Takimoto, S., Fang, B., Burton, D.W., Moosa, A.R., Hoffman, R.M., Bouvet, M. Gene Therapy of Pancreatic Cancer With Green Fluorescent Protein and Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Fusion Gene Expression Driven by a Human Telomerase Reverse Transcriptase Promoter. Annals of Surgical Oncology, 10(7): 762-772, 2003. [PDF] 

Head and Neck

  1. Lee, S.E., Bairstow, S.F., Werling, J.O., Chaubal, M.V., Lin, L., Murphy, M.A., Diorio, J. P., Gass, J., Rabinow, B., Wang, X., Zhang, Y., Yang, Z., and Hoffman, R.M. Paclitaxel nanosuspensions for targeted chemotherapy – nanosuspension preparation, characterization, and use. Pharm. Dev.Technol. 19, 438-453, 2014.

Kidney

  1. An, Z., Jiang, P., Wang, X., Moossa, A.R., and Hoffman, R.M. Development of a high metastatic orthotopic model of human renal cell carcinoma in nude mice: Benefits of fragment implantation compared to cell-suspension injection.  Clinical & Experimental Metastasis 17, 265-270, 1999. [PDF]

Liver

  1. Sun, F.X., Tang, Z.Y., Liu, K.D., Ye, S.L., Xue, Q., Gao, D.M., and Ma, Z.C.  Establishment of a metastatic model of human hepatocellular carcinoma in nude mice via orthotopic implantation of histologically intact tissues. Int. J. Cancer 66, 239-243, 1996. [PDF] 

  2. Sun, F-X., Tang, Z-Y., Liu, K-D., Xue, Q., Gao, D-M., Yu, Y-Q., Zhou, X-D., Ma, Z-C.  Metastatic models of human liver cancer in nude mice orthotopically constructed by using histologically intact patient specimens.  J. Cancer Res. Clin. Oncol. 122, 397-402, 1996. [PDF]

  3. Rashidi, B., An, Z., Sun, F-X, Li, X-M., Tang, Z.Y., Moossa, A.R., and Hoffman, R.M.  Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice.  Int. J. Cancer 91, 231-235, 2001. [PDF]  

  4. Wu, M., Mazarchuk, R., Chaudhary, N.D., Spernyak, J., Veith, J., Pera, P., Greco, W., Hoffman, R.M., Kobayashi, T., and Bernacki, R.J. High Resolution Magnetic Resonance Imaging of the Efficacy of the Cytosine Analogue 1-[2-C-Cyano-2-deoxy-b-D-arabino-pentofuranosyl]-N4 - palmitoyl Cytosine (CS-682) in a Liver-Metastasis Athymic Nude Mouse Model. Cancer Res. 63, 2477-2482, 2003. [PDF] 

  5. Suetsugu, S., Osawa, Y., Nagaki, M., Moriwaki, H., Saji, S., Bouvet, M., and Hoffman, R.M.  Simultaneous color-coded imaging to distinguish cancer “stem-like” and non-stem cells in the same tumor.  J. Cell. Biochem. 111, 1035-1041, 2010.

  6. Wang, M., Zhang, X., Xiong, X., Yang, Z., Sun, Y., Yang, Z., Hoffman, R.M., and Liu, Y.  Efficacy of the Chinese Traditional Medicinal Herb Celastrus orbiculatus Thunb on human hepatocellular carcinoma in an orthotopic fluorescent nude mouse model.  Anticancer Res. 32, 1213-1220, 2012.

  7. Momiyama, M., Kumamoto, T., Suetsugu, A., Kishimoto, H., Chishima, T., Tanaka, K., Akiyama, H., Ichikawa, Y., Bouvet, M., Endo, I., and Hoffman, R.M.  Major liver resection stimulates stromal recruitment and metastasis compared with repeated minor resection.  J. Surg. Res. 178, 280-287, 2012.

  8. He, G., Dhar, D., Nakagawa, H., Font-Burgada, J., Ogata, H., Jiang, Y., Shalapour, S., Seki, E., Yost, S.E., Jepsen, K., Frazer, K.A., Harismendy, O., Hatziapostolou, M.,Iliopoulos, D., Suetsugu, A., Hoffman, R.M., Tateishi, R., Koike, K., Karin, M. Identification of Liver Cancer Progenitors Whose Malignant Progression Depends on Autocrine IL-6 Signaling. Cell 155, 384-396, 2013.

Lung

  1. Wang, X., Fu, X., and Hoffman, R.M.  A new patient-like metastatic model of human lung cancer constructed orthotopically with intact tissue via thoracotomy in immunodeficient mice.  Int. J. Cancer 51, 992-995, 1992. [PDF]

  2. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Kase, S., Tanino, H., Nishibori, K., Saikawa, Y., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  Orthotopic reconstitution of human small-cell lung carcinoma after intravenous transplantation in SCID mice.  Anticancer Res. 12, 1407-1410, 1992.[PDF]

  3. Wang, X., Fu, X., and Hoffman, R.M.  A patient-like metastasizing model of human lung adenocarcinoma constructed via thoracotomy in nude mice.  Anticancer Res. 12, 1399-1402, 1992. [PDF]

  4. Wang, X., Fu, X., Kubota, T., and Hoffman, R.M.  A new patient-like metastatic model of human small-cell lung cancer constructed orthotopically with intact tissue via thoracotomy in nude mice.  Anticancer Res. 12, 1403-1406, 1992. [PDF]

  5. Kuo, T-H., Kubota, T., Watanabe, M., Furukawa, T., Kase, S., Tanino, H., Saikawa, Y., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  Site-specific chemosensitivity of human small-cell lung carcinoma growing orthotopically compared to subcutaneously in SCID mice: the importance of orthotopic models to obtain relevant drug evaluation data.  Anticancer Res. 13, 627-630, 1993. [PDF]

  6. Astoul, P., Wang, X., and Hoffman, R.M.  "Patient-like" nude- and SCID-mouse models of human lung and pleural cancer (Review).  Int. J. Oncology  3, 713-718, 1993. [PDF]

  7. Astoul, P., Colt, H.G., Wang, X., and Hoffman, R.M.  A “patient-like” nude mouse model of parietal pleural human lung adenocarcinoma.  Anticancer Res. 14, 85-92, 1994.

  8. Astoul, P., Colt, H.G., Wang, X., Boutin, C. and Hoffman, R.M.  A “patient-like” nude mouse metastatic model of  advanced human pleural cancer.  Journal of Cellular Biochemistry 56, 9-15, 1994.[PDF] 

  9. Astoul, P., Wang, X., Colt, H.G., Boutin, C., and Hoffman, R.M.  A patient-like human malignant pleural mesothelioma nude-mouse model.  Oncology Reports 3, 483-487, 1996.

  10. Colt, H.G., Astoul, P., Wang, X., Yi, E.S., Boutin, C., and Hoffman, R.M.  Clinical course of human epithelial-type malignant pleural mesothelioma replicated in an orthotopic-transplant nude mouse model.   Anticancer Res. 16, 633-640, 1996. [PDF] 

  11. An, Z., Wang, X., Astoul, P., Danays, T., Moossa, A.R., and Hoffman, R.M.  Interferon gamma is highly effective against orthotopically-implanted human pleural adenocarcinoma in nude mice.  Anticancer Res. 16, 2545-2551, 1996. [PDF]

  12. Olbina, G., Cieslak, D., Ruzdijic, S., Esler, C., An, Z., Wang, X., Hoffman, R., Seifert, W., and Pietrzkowski, Z. Reversible inhibition of IL-8 receptor B mRNA expression and proliferation in non-small cell lung cancer by antisense oligonucleotides.  Anticancer Res. 16, 3525-3530, 1996. [PDF]

  13. Yang, M., Hasegawa, S., Jiang, P., Wang, X., Tan, Y., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M.  Widespread skeletal metastatic potential of human lung cancer revealed by green fluorescent protein expression.  Cancer Res. 58, 4217-4221, 1998. [PDF]

  14. Rashidi, B., Yang, M., Jiang, P., Baranov, E., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M.  A highly metastatic Lewis lung carcinoma orthotopic green fluorescent protein model.  Clinical & Experimental Metastasis 18, 57-60, 2000. [PDF] 

  15. Hoffman, Robert M.  Metastatic Mouse Models of Lung Cancer.  In: Driscoll, B., ed.  Methods in Molecular Medicine, Vol. 74: Lung Cancer, Vol. 1: Molecular Pathology Methods and Reviews.  Totowa, NJ: Humana Press, 457-464, 2002.. [PDF]

  16. Yamamoto, N., Yang, M., Jiang, P., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Real-time GFP imaging of spontaneous HT-1080 fibrosarcoma lung metastases.  Clin. Exp. Metastasis 20, 181-185, 2003. [PDF] 

  17. Bobek, V., Boubelik, M., Fiserova, A., L'uptovcova, M., Vannucci, L., Kacprzak, G., Kolodzej, J., Majevski, A.M., and Hoffman R.M. Anticoagulant drugs increase natural killer cell activity in lung cancer. Lung Cancer 47, 215-223, 2005. 

  18. Hoffman, R.M. Noninvasive imaging for evaluation of the systemic delivery of capsid-modified adenovirus in an orthotopic model of advanced lung cancer. Cancer 109, 1213-1214, 2007. [PDF]

  19. Zhang, C., Lv, F., Zhou, L., Li, X., Wu, X-X., and Hoffman, R.M.  Effect of verapamil on the expression of EGFR and NM23 in A549 humanlung cancer cells.  Anticancer Res. 29, 27-32, 2009.

  20. Kimura, H., Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Kishimoto, H., Bouvet, M., and Hoffman, R.M.  Real-time imaging of single cancer-cell dynamics of lung metastasis.  J. Cell. Biochem. 109, 58-64, 2010.

  21. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., Gurlich, R., and Hoffman, R.M.  Tail spontaneous metastatic mouse model: Comparison of metastatic potential of orthotopic and heterotopic models imaged by GFP and RFP protein.  In Vivo 25, 849-852, 2011.

  22. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., and Hoffman, R.M.  Development of new spontaneous metastatic heterotopic model of Lewis lung carcinoma imaged by GFP Expression.  Cancer Invest. 29, 692-695, 2011.

  23. Zhang, Y., Zhang, N., Su, S., Hoffman, R.M., Zhao, M. Salmonella typhimurium A1-R tumor targeting in immunocompetent mice is enhanced by a Traditional Chinese Medicine herbal mixture. Anticancer Res. 33, 1837-1843, 2013.

  24. Rashidi, B., Moossa, A.R., and Hoffman, R.M. Specific route mapping visualized with GFP of single-file streaming contralateral and systemic metastasis of Lewis lung carcinoma cells beginning within hours of orthotopic implantation. J. Cell. Biochem 114, 1738-1743, 2013.

Lymphoma

  1. Schmitt, C.A., Fridman, J.S., Yang, M., Baranov, E., Hoffman, R.M. and Lowe, S.W.  Dissecting p53 tumor suppressor functions in vivo.  Cancer Cell 1, 289-298, 2002.

  2. Schmitt, C.A., Yang, M., Fridman, J.S., Baranov, E., Hoffman, R.M., and Lowe, S.W.  A senescence program controlled by p53 and p16INK4acontributes to the outcome of cancer therapy.  Cell 109, 335–346, 2002.

Melanoma

  1. Yang, M., Jiang, P., An, Z., Baranov, E., Li, L., Hasegawa, S., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M. Genetically fluorescent melanoma bone and organ metastasis models. Clinical Cancer Research 5, 3549-3559, 1999. [PDF] 

  2. Yang, M., Baranov, E., Jiang, P., Sun, F-X., Li, X-M., Li, L., Hasegawa, S., Bouvet, M., Al-Tuwaijri, M., Chishima, T., Shimada, H., Moossa, A.R., Penman, S., Hoffman, R.M. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases. Proc. Natl. Acad. Sci. USA 97, 1206-1211, 2000. [PDF]

  3. Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M. Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells. Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003. [PDF] 

  4. Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., and Hoffman, R.M. Hair follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin. Cancer Res. 65, 2337-2343. 2005.[PDF]

  5. Zhang, Q., Fan, H., Shen, J., Hoffman, R.M., and Xing, H.R. Human breast cancer cell lines co-express neuronal, epithelial, and melanocytic differentiation markers in vitro and in vivo. PLoS One 5, e9712, 2010. DOI: 10.1371/journal.pone.0009712.

  6. 6. Tsai, M-H., Aki, R., Amoh, Y., Hoffman, R.M., Katsuoka, K., Kimura, H., Lee, C., and Chang, C-H. GFP-fluorescence-guided UVC irradiation inhibits melanoma growth and angiogenesis in nude mice. Anticancer Research 30, 3291-3294, 2010.

  7. Bobek, V., Kolostova, K., Pinterova, D., Kacprzak, G., Adamiak, J., Kolodzej, J., Boubelik, M., Kubecova, M., and Hoffman, R.M. A clinically-relevant, syngeneic model of spontaneous, high-metastatic B16 mouse melanoma. Anticancer Research 30, 4799-4804, 2010

  8. Bobek, V., Kolostova, K., Pinterova, D., Boubelik, M., Gurlich, R., and Hoffman, R.M.  Tail spontaneous metastatic mouse model: Comparison of metastatic potential of orthotopic and heterotopic models imaged by GFP and RFP protein.  In Vivo 25, 849-852, 2011.

  9. Binder, D.C., Engels, B., Arina, A., Yu, P., Stauch, J.M., Fu, Y-X, Karrison, T., Burnette, B., Idel, C., Zhao, M., Hoffman, R.M., Munn, D.H., Rowley, D.A., and Schreiber, H. Antigen-specific bacterial vaccine combined with anti-PD-L1 rescues dysfunctional endogenous T cells to reject long-established cancer. Cancer Immunol. Res., 1, 123-133, 2013.

Multiple Myeloma

  1. Mitsiades, C.S., Mitsiades, N.S., Bronson, R.T., Chauhan, D., Munshi, N., Treon, P.S., Maxwell, C.A., Pilarski, L., Hideshima, T., Hoffman,R.M. Fluorescent Imaging of Multiple Myeloma Cells in a Clinically Relevant SCID/NOD in vivo Model: Biological and Clinical Implications. Cancer Research 63, 6689-6696, 2003. [PDF].

Nasopharyngeal Carcinoma

  1. Liu, T., Ding, Y., Xie, W., Li, Z., Bai, X., Li, X., Fang, W., Ren, C., Wang, S., Hoffman, R.M., and Yao, K.  An imageable metastatic treatment model of nasopharyngeal carcinoma.  Clin. Cancer Res. 13,     3960-3967, 2007.[PDF]

Osteosarcoma

  1. Kimura, H., Tome, Y., Momiyama, M., Hayashi, K., Tsuchiya, H., Bouvet, M., and Hoffman, R.M.  Imaging the inhibition by anti-b1 integrin antibody of lung seeding of single osteosarcoma cells in live mice.  Int. J. Cancer 131, 2027-2033, 2012.  DOI: 10.1002/ijc.27475.

  2. Uehara, F., Tome, Y., Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Bouvet, M., Maehara, H., Kanaya, F., and Hoffman, R.M. A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res. 33, 1361-1366, 2013.

  3. Wolf, K., te Lindert, M., Krause, M., Alexander, S., te Riet, J., Willis, A.L., Hoffman, R.M., Figdor, C.G., Weiss, S.J., and Friedl, P. Physical limits of cell migration: Control by ECM space and nuclear deformation and tuning by proteolysis and traction force. J. Cell. Biol. 201, 1069-1084, 2013.

  4. Tome, Y., Sugimoto, N., Yano, S., Momiyama, M., Mii, S., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Research 33, 3021-3025.

Ovarian

  1. Fu, X., and Hoffman, R.M.  Human ovarian carcinoma metastatic models constructed in nude mice by orthotopic transplantation of histologically-intact patient specimens.  Anticancer Res. 13, 283-286, 1993.[PDF]

  2. Astoul, P., Colt, H.G., Wang, X., and Hoffman, R.M.  Metastatic human pleural ovarian cancer model constructed by orthotopic implantation of fresh histologically-intact patient carcinoma in nude mice.  Anticancer Res. 13, 1999-2002, 1993. [PDF]

  3. Kiguchi, K., Kubota, T., Aoki, D., Udagawa, Y., Yamanouchi, S., Saga, M., Amemiya, A., Nozawa, S., Moossa, A.R., and Hoffman, R.M.  A patient-like orthotopic implantation nude mouse model of highly metastatic human ovarian cancer. Clinical & Experimental Metastasis 16, 751-756, 1998. [PDF]

  4. Steinkamp, M.P., Winner, K.K., Davies, S., Muller, C., Zhang, Y., Hoffman, R.M., Shirinifard, A., Moses, M., Jiang, Y., and Wilson, B.S. Ovarian tumor attachment, invasion, and vascularization reflect unique microenvironments in the peritoneum: insights from xenograft and mathematical models. Frontiers in Oncology, published online, 2013. DOI: 10.3399/fonc.2013.00097.

  5. Lee, S.E., Bairstow, S.F., Werling, J.O., Chaubal, M.V., Lin, L., Murphy, M.A., Diorio, J. P., Gass, J., Rabinow, B., Wang, X., Zhang, Y., Yang, Z., and Hoffman, R.M. Paclitaxel nanosuspensions for targeted chemotherapy – nanosuspension preparation, characterization, and use. Pharm. Dev.Technol. 19, 438-453, 2014.

Pancreas

  1. Fu, X., Guadagni, F., and Hoffman, R.M.  A metastatic nude-mouse model of human pancreatic cancer constructed orthotopically from histologically intact patient specimens.  Proc. Natl. Acad. Sci. USA 89, 5645-5649, 1992. [PDF] 

  2. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M.  A novel "patient-like" treatment model of human pancreatic cancer constructed using orthotopic transplantation of histologically intact human tumor tissue in nude mice.  Cancer Res. 53, 3070-3072, 1993.

  3. An, Z., Wang, X., Kubota, T., Moossa, A.R., Hoffman, R.M.  A clinical nude mouse metastatic model for highly malignant human pancreatic cancer.  Anticancer Res. 16, 627-631, 1996.

  4. Tomikawa, M., Kubota, T., Matsuzaki, S.W., Takahasi, S., Kitajima, M., Moossa, A.R., and Hoffman, R.M.  Mitomycin C and cisplatin increase survival in a human pancreatic cancer metastatic model.  Anticancer Res. 17, 3623-3626, 1997.

  5. Bouvet, M., Yang, M., Nardin, S., Wang, X., Jiang, P., Baranov, E., Moossa, A.R., Hoffman, R.M.  Chronologically-specific metastatic targeting of human pancreatic tumors in orthotopic models.  Clinical & Experimental Metastasis 18, 213-218, 2000.

  6. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M.  Antimetastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model. Clinical & Experimental Metastasis 18, 379-384, 2001.[PDF] 

  7. Bouvet, M., Wang, J-W., Nardin, S.R., Nassirpour, R., Yang, M., Baranov, E., Jiang, P., Moossa, A.R., and Hoffman, R.M.  Real-time optical imaging of primary tumor growth and multiple metastatic events in a pancreatic cancer orthotopic model.  Cancer Research 62, 1534-1540, 2002. [PDF]

  8. Bouvet, M., Nardin, S.R., Burton, D.W., Lee, N.C., Yang, M., Wang, X., Baranov, E., Behling, C., Moossa, A.R., Hoffman, R.M., and Deftos L.J.  Parathyroid hormone-related protein as a novel tumor marker in pancreatic adenocarcinoma.  Pancreas 24, 284-290, 2002.

  9. Sun, F-X., Tohgo, A., Bouvet, M., Yagi, S., Nassirpour, R., Moossa, A.R., and Hoffman, R.M.  Efficacy of camptothecin analog DX-8951f (Exatecan Mesylate) on human pancreatic cancer in an orthotopic metastatic model.  Cancer Research 63, 80-85, 2003. [PDF] 

  10. Katz, M., Takimoto, S., Spivac, D., Moossa, A.R., Hoffman, R.M., Bouvet, M. A novel red fluorescent protein orthotopic pancreatic cancer model for the preclinical evaluation of chemotherapeutics. J.Surg. Res. 113, 151-160, 2003.[PDF] 

  11. Katz, M.H., Bouvet, M., Takimoto, S., Spivac, D., Moossa, A.R., Hoffman, R.M. Selective antimetastatic activity of cytosine analog CS-682 in a red fluorescent protein orthotopic model of pancreatic cancer. Cancer Res. 63, 5521-5525, 2003.[PDF] 

  12. Tsuji, K., Yamauchi, K., Jiang, P., Yang, M., and Hoffman, R.M. Common bile duct injection as a novel method for establishing RFP-expressing human pancreatic cancer in nude mice. Journal of the Pancreas 7, 193-199, 2006. 

  13. Katz, M., Spivac, D., Takimoto, S., Bouvet, M., Moosa, A.R., R.M. Hoffman. An imageable highly metastatic orthotopic red fluorescent protein model of pancreatic cancer. Clin. Exp. Metastasis 21, 7-12, 2004.[PDF]

  14. Katz, M.H., Bouvet, M., Takimoto, S., Spivack, D., Moosa, A.R., and Hoffman, R.M. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer. Cancer Research 64, 1828-1833, 2004. [PDF] 

  15. Bouvet, M., Spernyak, J., Katz, M.H., Mazurchuk, R.V., Takimoto, S., Bernacki, R., Rustum, Y.M., Moossa, A.R., and Hoffman, R.M. High correlation of whole- body red fluorescent protein imaging and magnetic resonance imaging on an orthotopic model of pancreatic cancer. Cancer Research 65, 9829-9833, 2005. [PDF]

  16. Amoh, Y., Li, L., Tsuji, K., Moosa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M. Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antioangiogenesis efficacy of gemcitabine. J. Surgical Research 132, 164-169, 2006. 

  17. Amoh, Y., Nagakura, C., Maitra, A., Moossa, A.R., Katsuoka, K., Hoffman, R.M., and Bouvet, M. Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.[PDF]

  18. Bouvet, M., Hoffman R.M. Preclinical fluorescent mouse models of pancreatic cancer. Proc. of SPIE 6449, 64490X-1 - 64490X-11, 2007.[PDF]

  19. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M. A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo. Neoplasia 9, 625-633, 2007.[PDF]

  20.  Tran Cao, H.S., Kimura, H., Kaushal, S., Snyder, C.S., Reynoso, J., Hoffman, R.M., and Bouvet, M.  The cyan fluorescent protein (CFP) transgenic mouse as a model for imaging pancreatic exocrine cells.   Journal of the Pancreas 10, 152-156, 2009.

  21. Tran Cao, H.S., Reynoso, J., Yang M., Kimura, H., Kaushal, S., Snyder, C.S., Hoffman, R.M., and Bouvet M. Development of the transgenic cyan fluorescent protein (CFP)-expressing nude mouse for “Technicolor” cancer imaging.  J. Cell. Biochem 107, 328-334, 2009.

  22. Snyder, C.S., Kaushal, S., Kono, Y., Tran Cao, H.S., Hoffman, R.M., and Bouvet, M.  Complementarity of ultrasound and fluorescence imaging in an orthotopic mouse model of pancreatic cancer.  BMC Cancer 9, 106, 2009.  DOI:  10.1117/1.3103783.

  23. Matsuo, Y., Raimondo, M., Woodward, T.A., Wallace, M.B., Gill, K.R., Tong, Z., Burdick, M.D., Yang, Z., Strieter, R.M., Hoffman, R.M., Guha, S.  CXC-chemokine/CXCR2 biological axis promotes angiogenesis in vitro and in vivo in pancreatic cancer.  Int. J. Cancer125, 1027-1037, 2009.

  24. Nagakura, C., Hayashi, K., Zhao, M., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M. Efficacy of a genetically-modified Salmonella typhimurium in an orthotopic human pancreatic cancer in nude mice.  Anticancer Res.29, 1873-1878, 2009.

  25. Zhou, J., Yu, Z., Zhao, S., Hu, L., Zheng, J., Yang, D., Bouvet, M., Hoffman, R.M.  Lentivirus-based DsRed-2-transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease.  J. Surg. Res. 157, 63-70, 2009.

  26. Bouvet, M., and Hoffman, R.M.  Clinically-relevant orthotopic metastatic models of pancreatic cancer imageable with fluorescent genetic reporters.  Minerva Chirurgica 64, 521-539, 2009.

  27. Logan-Collins, J., Thomas, R.M., Yu, P., Jaquish, D., Mose, E., French, R., Stuart, W., McClaine, R., Aronow, B., Hoffman, R.M., Waltz, S.E., and Lowy, A.M.  Silencing of RON receptor signaling promotes apoptosis and gemcitabine sensitivity in pancreatic cancers.  Cancer Res. 70, 1130-40, 2010.  DOI: 10.1158/0008-5412.CAN-09-0761.

  28. Murphy, E.A., Shields, D.J., Stoletov, K., Dneprovskaia, E., McElroy, M., Greenberg, J.I., Lindquist, J., Acevedo, L.M., Anand, S., Majeti, B.K., Tsigelny, I., Saldanha, A., Walsh, B., Hoffman, R.M., Bouvet, M., Klemke, R.L., Vogt, P.K., Arnold, L., Wrasidlo, W., and Cheresh, D.A.  Disruption of angiogenesis and tumor growth with an orally active drug that stabilizes the inactive state of PDGFRb/B-RAF.  Proc. Natl. Acad. Sci. USA 107, 4299-4304, 2010.  DOI: 10.1073/pnas.0909299107.

  29.  Tran Cao, H.S., Bouvet, M., Kaushal, S., Keleman, A., Romney, E., Kim, G., Fruehauf, J., Imagawa, D.K., Hoffman, R.M., and Katz, M.  Metronomic gemcitabine in combination with Sunitinib inhibits multisite metastasis and increases survival in an orthotopic model of pancreatic cancer.  Molecular Cancer Therapeutics 9, 2068-2078, 2010.  (Highlights Section. Molecular Cancer Therapeutics9, 1929, 2010).

  30. Tran Cao, H., Kaushal, S., Lee, C., Snyder, C.S., Thompson, K.J., Horgan, S., Talamini, M.A., Hoffman, R.M., and Bouvet, M. Fluorescence laparoscopy imaging of pancreatic tumor progression in an orthotopic mouse model. Surg. Endosc. 25, 48-54, 2011.

  31. Tran Cao, H.S., Kaushal, S., Menen, R.S., Metildi, C.A., Lee, C., Snyder, C.S., Talamini, M.A., Hoffman, R.M., and Bouvet, M.  Submillimeter-resolution fluorescence laparoscopy of pancreatic cancer in a carcinomatosis mouse model visualizes metastases not seen with standard laparoscopy.  J. Laparoendosc. Adv. Surg. Tech. A 21, 485-489, 2011 (Cover story).

  32. Grzesiak, J.J., Tran Cao, H.S., Burton, D.W., Kaushal, S., Vargas, F., Clopton, P., Snyder, C.S., Deftos, L.J., Hoffman, R.M., and Bouvet, M.  Knockdown of the β1 integrin subunit reduces primary tumor growth and inhibits pancreatic cancer metastasis.  International Journal of Cancer129, 2905-2915, 2011.

  33. Hassanein, M.K., Suetsugu, A., Saji, S., Moriwaki, H., Bouvet, M., Moossa, A.R., and Hoffman, R.M.  Stem-like and non-stem human pancreatic cancer cells distinguished by morphology and metastatic behavior.  Journal of Cellular Biochemistry 112, 3549-3554, 2011 (Cover story).

  34. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M.  Imageable fluorescent metastasis resulting in transgenic GFP mice orthotopically implanted with human-patient primary pancreatic cancer specimens.  Anticancer Research 32, 1175-1180, 2012.

  35. Kelber, J.A., Reno, T., Kaushal, S., Metildi, C., Wright, T., Stoletov, K., Weems, J.M., Park, F.D., Mose, E., Wang, Y., Hoffman, R.M., Lowy, A.M., Bouvet, M., and Klemke, R.L.  KRas induces a Src/PEAK1/ErbB2 kinase amplification loop that drives metastatic growth and therapy resistance in pancreatic cancer.  Cancer Research 72, 2554-2564,2012.

  36. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Moriwaki, H., Bouvet, M., Saji, S., and Hoffman, R.M.  Multi-color palette of fluorescent proteins for imaging the tumor microenvironment of orthotopic tumorgraft mouse models of clinical pancreatic cancer specimens.  J. Cell. Biochem. 113, 2290-2295, 2012.

  37. Metildi, C.A., Kaushal, S., Lee, C., Hardamon, C.R., Snyder, C.S., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M.  An LED light source and novel fluorophore combinations improve fluorescence laparoscopic detection of metastatic pancreatic cancer in orthotopic mouse models.  J. Am. Coll. Surg. 214, 997-1007, 2012.

  38. Bouvet, M., and Hoffman, R.M.  In vivo imaging of pancreatic cancer with fluorescent proteins in mouse models (Chap. 4).  In  In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols.  Hoffman, R.M., ed.  Methods in Molecular Biology, Vol. 872.  Walker, John M., series ed.  Humana Press (Springer Science+Business Media New York), 2012.

  39. Yu, Z., Zhou, J., and Hoffman, R.M.  Lentivirus-based DsRed-2-transfected pancreatic cancer cells for deep in vivo imaging of metastatic disease (Chap. 5).  In: In Vivo Cellular Imaging Using Fluorescent Proteins: Methods and Protocols.  Hoffman, R.M., ed.  Methods in Molecular Biology, Vol. 872.  Walker, John M., series ed.  Humana Press (Springer Science+Business Media New York), 2012.

  40. Suetsugu, A., Katz, M., Fleming, J., Truty, M., Thomas, R., Saji, S., Moriwaki, H., Bouvet, M., and Hoffman, R.M.  Non-invasive fluorescent-protein imaging of orthotopic pancreatic-cancer-patient tumorgraft progression in nude mice. Anticancer Research 32, 3063-3068, 2012.

  41. Tran Cao, H.S., Kaushal, S., Metildi, C.A., Menen, R.S., Lee, C., Snyder, C.S., Messer, K., Pu, M., Luiken, G.A., Talamini, M.A., Hoffman, R.M., and Bouvet, M.  Tumor-specific fluorescence antibody imaging enables accurate staging laparoscopy in an orthotopic model of pancreatic cancer.  Hepatogastroenterology 59(118), 1994-1999, 2012.  DOI: 10.5754/hge11836.

  42. Shi, Y., Tong, M., Wu, Y., Yang, Z., Hoffman, R.M., Zhang, Y., Tian, Y., Qi, M., Lin, Y., Liu, Y., Dai, L., Sun, Y., and Wang, Z.  VEGF-C ShRNA inhibits pancreatic cancer growth and lymphangiogenesis in an orthotopic fluorescent nude mouse model.  Anticancer Research 33, 409-417, 2013.

  43. Snyder, C.S., Harrington, A.R., Kaushal, S., Mose, E., Lowy, A.M., Hoffman, R.M., and Bouvet, M. A dual-color genetically engineered mouse model for multispectral imaging of the pancreatic microenvironment. Pancreas 42, 952-958, 2013.

  44. Zhang, L., Wu, C., Zhang, Y., Liu, F., Zhao, M., Bouvet, M., and Hoffman, R.M. Efficacy comparison of traditional Chinese medicine LQ versus gemcitabine in a mouse model of pancreatic cancer. J. Cell. Biochem. 114, 2131-2137, 2013.

  45. Hiroshima, Y., Zhao, M., Zhang, Y., Maawy, A., Hassanein, M.K., Uehara, F., Miwa, S., Yano, S., Momiyama, M., Suetsugu, A., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., and Hoffman, R.M. Comparison of efficacy of Salmonella typhimurium A1-R and chemotherapy on stem-like and non-stem human pancreatic cancer cells. Cell Cycle 12, 2774-2780, 2013.

  46. Miwa, S., Yano, S., Hiroshima, Y., Tome, Y., Uehara, F., Mii, S., Efimove, E.V., Kimura, H., Hayashi, K., Tsuchiya, H., Hoffman, R.M. Imaging UVC-induced DNA damage response in models of minimal cancer. J. Cell. Biochem. 114, 2493-2499, 2013.

  47. Metildi, C.A., Kaushal, S., Hoffman, R.M., and Bouvet, M. In vivo serial selection of human pancreatic cancer cells in orthotopic mouse models produces high metastatic variants irrespective of Kras status. J. Surg. Res. 184, 290-298, 2013.

  48. Maawy, A.A., Hiroshima, Y., Kaushal, S., Luiken, G.A., Hoffman, R.M., and Bouvet, M. Comparison of a chimeric anti-carcinoembryonic antigen antibody conjugated with visible or near-infrared fluorescent dyes for imaging pancreatic cancer in orthotopic nude mouse models. J. Biomed. Optics 18, 126016, 2013.

Prostate

  1. Fu, X., Herrera, H., and Hoffman, R.M.  Orthotopic growth and metastasis of human prostate carcinoma in nude mice after transplantation of histologically intact tissue.  Int. J. Cancer 52, 987-990, 1992. [PDF] 

  2. An, Z., Wang, X., Geller, J., Moossa, A.R., and Hoffman, R.M.   Surgical orthotopic implantation allows high lung and lymph node metastatic expression of human prostate carcinoma cell line PC- 3 in nude mice.  The Prostate 34, 169-174, 1998.

  3. Yang, M., Jiang, P., Sun, F.X., Hasegawa, S., Baranov, E., Chishima, T., Shimada, H., Moossa, A.R., and Hoffman, R.M.  A fluorescent orthotopic bone metastasis model of human prostate cancer.  Cancer Res. 59, 781-786, 1999. [PDF] 

  4. Wang, X., An, Z., Geller, J., and Hoffman, R.M.  High malignancy orthotopic nude mouse model of human prostate cancer LNCaP.  The Prostate 39, 182-186, 1999.  

  5. Glinskii, A.B., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M., Glinsky, G.V. Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models. Cancer Res. 63, 4239-4243, 2003.[PDF] 

  6. Glinsky, G.V., Glinskii, A.B., Stephenson A.J., Hoffman, R.M., Gerald W.L. Gene expression profiling predicts clinical outcome of prostate cancer. J. Clin. Invest. 113, 913-923, 2004 [PDF]

  7. Yang, M., Jiang, P., Yamamoto, N., Li, L., Geller, J., Moossa, A.R., and Hoffman, R.M. Real-time whole-body imaging of an orthotopic metastatic prostate cancer model expressing rad fluorescent protein. The Prostate 62, 374-379,2005. DOI #10.1002/pros.20125. [PDF]

  8. Burton, D.W., Geller, J., Yang, M., Jiang, P., Barken, I., Hastings, R.H., Hoffman, R.M., and Deftos, L.J. Monitoring of skeletal progression of prostate cancer by GFP imaging, X-ray, and serum OPG and PTHrP. The Prostate 62, 275-281, 2005. DOI: 10.1002/pros.20146. [PDF]

  9. Deftos, L.J., Barken, I., Burton, D.W., Hoffman, R.M., and Geller, J. Direct evidence that PTHrP expression promotes prostate cancer progression in bone. Biochem. Biophys. Res. Commun. 327, 468-472, 2005. [PDF]

  10. Berezovskaya, O., Schimmer, A.D., Glinskii, A.B., Pinilla, C., Hoffman, R.M., Reed, J.C., and Glinky, G.V. Increased _expression of apoptosis inhibitor protein XIAP contributes to resistance to anoikis of human prostate cancer metastasis precursor cells. Cancer Research 65, 2378-2386, 2005. [PDF]

  11. Glinsky, G.V., Glinski, A.B., Berezovskaya, O., Smith, B.A., Jiang, P., Li, X-M., Yang, M., Hoffman, R.M. Dual color-coded imaging of viable circulating prostate carcinoma cells reveals genetic exchange between tumor cells in vivo, contributing to highly metastatic phenotypes. Cell Cycle 5, 191-197, 2006.

  12. Yang, M., Burton, D.W., Geller, J., Hillegonds, D., Hastings, R.H., Deftos, L.J., and Hoffman, R.M. The bisphosphonate olpadronate inhibits skeletal prostate cancer progression in a green fluorescent protein nude mouse model. Clinical Cancer Research 12, 2602-2606, 2006. [PDF] 

  13. Berezovska, O.P., Glinskii, A.B., Yang, Z., Li, X.M., Hoffman, R.M., and Glinsky, G.V. Essential role for activation of the Polycomb Group (PcG) protein chromatin silencing pathway in metastatic prostate cancer. Cell Cycle 5, 1886-1901, 2006. [PDF] 

  14. Hoffman, R.M. Orthotopic metastatic mouse models of prostate cancer. In: Metastasis of Prostate Cancer, pp. 143-169. Ablin, R.J. and Mason, M.D., eds. Kluwer Academic Publishers, 2007.[PDF] 

  15. Zhang, Q., Yang, M., Shen, J., Gerhold, L.M., Hoffman, R.M., Xing, H.R.  The role of the intravascular microenvironment in spontaneous metastasis development.  International Journal of Cancer 126, 2534-2541, 2010.

  16. Kolostova, K., Pinterova, D., Hoffman, R.M., and Bobek, V.  Circulating human prostate cancer cells from an orthotopic mouse model rapidly captured by immunomagnetic beads and imaged by GFP expression.  Anticancer Res. 31, 1535-1539, 2011.

  17. Menen, R.S., Pinney, E., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M.  A rapid imageable in vivometastasis assay for circulating tumor cells.  Anticancer Res. 31, 3125-3128, 2011.

  18. Menen, R., Pinney, E., Hassanein, M.K., Kolostova, K., Bobek, V., Suetsugu, A., Zhang, N., Bouvet, M., and Hoffman, R.M.  Inhibition of metastasis of circulating human prostate cancer cells in the chick embryo by an extracellular matrix produced by foreskin fibroblasts in culture.  Anticancer Research 32, 1573-1577, 2012.

  19. Menen, R., Zhao, M., Zhang, L., Hassanein, M.K., Bobek, V., Kolostova, K., Bouvet, M., and Hoffman, R.M.  Comparative chemosensitivity of circulating human prostate cancer cells and primary cancer cells.  Anticancer Research 32, 2881-2884, 2012.

  20. Ongkeko, W.M., Burton, D., Kiang, A., Abhold, E., Kuo, S.Z., Rahimy, E., Yang, M., Hoffman, R.M., Wang-Rodriguez, J., Deftos, L.J. Parathyroid hormone related-protein promotes epithelial-to-mesenchymal transition in prostate cancer. PLoS One 9, e85803, 2014.

Rectal

  1. Kishimoto, H., Momiyama, M., Aki, R., Kimura, H., Suetsugu, A., Bouvet, M., Fujiwara, T., and Hoffman, R.M. Development of a clinically-precise mouse model of rectal cancer. PLoS One 11, e79453, 2013.

Sarcoma

  1. Yamamoto, N., Yang, M., Jiang, P., Xu, M., Tsuchiya, H., Tomita, K., Moossa, A.R., and Hoffman, R.M.  Real-time imaging of individual color-coded metastatic colonies in vivo.  Clin. Exp. Metastasis 20(7), 633-638, 2003.[PDF]   

  2. Kimura, H., Tome, Y., Momiyama, M., Hayashi, K., Tsuchiya, H., Bouvet, M., and Hoffman, R.M. Imaging the inhibition by anti-b1 integrin antibody of lung seeding of single osteosarcoma cells in live mice. Int. J. Cancer 131, 2027-2033, 2012.

  3. Uehara, F., Tome, Y., Yano, S., Miwa, S., Mii, S., Hiroshima, Y., Bouvet, M., Maehara, H., Kanaya, F., and Hoffman, R.M. A color-coded imaging model of the interaction of αv integrin-GFP expressed in osteosarcoma cells and RFP expressing blood vessels in Gelfoam® vascularized in vivo. Anticancer Res. 33, 1361-1366, 2013.

  4. Tome, Y., Sugimoto, N., Yano, S., Momiyama, M., Mii, S., Maehara, H., Bouvet, M., Tsuchiya, H., Kanaya, F., and Hoffman, R.M. Real-time imaging of αv integrin molecular dynamics in osteosarcoma cells in vitro and in vivo. Anticancer Research 33, 3021-3025.

  5. Tome, Y., Kimura, H., Maehara, H., Sugimoto, N., Bouvet, M., Tsuchiya, H., Kanaya, F., Hoffman, R.M. High lung-metastatic variant of human osteosarcoma cells, selected by passage of lung metastasis in nude mice, is associated with increased expression of αvβ3 integrin. Anticancer Research 33, 3623-3628, 2013

  6. Miwa, S., Yano, S., Tome, Y., Sugimoto, N., Hiroshima, Y., Uehara, F., Mii, S., Kimura, H., Hayashi, K., Efimova, E.V., Fujiwara, T., Tsuchiya, H., and Hoffman, R.M. Dynamic color-coded fluorescence imaging of the cell-cycle phase, mitosis, and apoptosis demonstrates how caffeine modulates cisplatinum efficacy. J. Cell. Biochem. 114, 2454-2460, 2013.

Stomach

  1. Furukawa, T., Fu, X., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M. Nude mouse metastatic models of human stomach cancer constructed using orthotopic implantation of histologically intact tissue.  Cancer Res. 53, 1204-1208, 1993. [PDF] 

  2. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M.  Orthotopic transplantation of histologically intact clinical specimens of stomach cancer to nude mice: Correlation of metastatic sites in mouse and individual patient donors.  Int. J. Cancer 53, 608-612, 1993. [PDF]

  3. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Kitajima, M., and Hoffman, R.M.  Differential chemosensitivity of local and metastatic human gastric cancer after orthotopic transplantation of histologically intact tumor tissue in nude mice.  Int. J. Cancer 54, 397-401, 1993. [PDF]

  4. Inada, T., Ichikawa, A., Kubota, T., Ogata, Y., Moossa, A.R., and Hoffman, R.M.  5-FU-induced apoptosis correlates with efficacy against human gastric and colon cancer xenografts in nude mice.  Anticancer Res. 17, 1965-1971, 1997. [PDF]

  5. Hasegawa, S., Yang, M., Chishima, T., Shimada, H., Moossa, A.R., Hoffman, R.M.  In vivo tumor delivery of the green fluorescent protein gene to report future occurrence of metastasis. Cancer Gene Therapy 7, 1336-1340, 2000. [PDF] 

  6. Kamiyama, M., Ichikawa, Y., Ishikawa, T., Chishima, T., Hasegawa, S., Hamaguchi, Y., Nagashima, Y., Miyagi, Y., Mitsuhashi, M., Hyndman, D., Hoffman, R.M., Ohki, S., and Shimada, H.  VEGF receptor antisense therapy inhibits angiogenesis and peritoneal dissemination of human gastric cancer in nude mice. Cancer Gene Therapy 9, 197-201, 2002.[PDF]

TCM

  1. Wang, M., Zhang, X., Xiong, X., Yang, Z., Sun, Y., Yang, Z., Hoffman, R.M., and Liu, Y.  Efficacy of the Chinese Traditional Medicinal Herb Celastrus orbiculatus Thunb on human hepatocellular carcinoma in an orthotopic fluorescent nude mouse model.  Anticancer Res. 32, 1213-1220, 2012.

  2. Hu, M., Zhao, M., An, C., Yang, M., Zhang, Y., Suetsugu, A., Tome, Y., Yano, S., Fu, Y., Hoffman, R.M., and Hu, K.  Real-time imaging of induction of apoptosis of human breast cancer cells by the traditional Chinese medicine herb Tubeimu.  Anticancer Res. 32, 2509-2514, 2012.

    3.

Thyroid

  1. Tran Cao, H.S., Kaushal, S., Snyder, C.S., Ongkeko, W.M., Hoffman, R.M., and Bouvet, M.  Real-time imaging of tumor progression in a fluorescent orthotopic mouse model of thyroid cancer.  Anticancer Research 30, 4415-4422, 2010.

  2. Murata, T., Mizushima, H., Chinen, I., Moribe, H., Yagi, S., Hoffman, R.M., Kimura, T., Yoshino, K., Ueda, Y., Enomoto, T., and Mekada, E.  HB-EGF and PDGF mediate reciprocal interactions of carcinoma cells with cancer-associated fibroblasts to support progression of uterine cervical cancers.  Cancer Res. 71, 6633-6642, 2011.

    3.

Drug Discovery

  1. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T.H., Kase, S., Saikawa, Y., Tanino, H., Teramoto, T., Ishibiki, K., Kitajima, M., and Hoffman, R.M.  Immunochemotherapy prevents human colon cancer metastasis after orthotopic onplantation of histologically-intact tumor tissue in nude mice.  Anticancer Res. 13, 287-291, 1993.

  2. Furukawa, T., Kubota, T., Watanabe, M., Kuo, T-H., Kitajima, M., and Hoffman, R.M.  Differential chemosensitivity of local and metastatic human gastric cancer after orthotopic transplantation of histologically intact tumor tissue in nude mice.  Int. J. Cancer 54, 397-401, 1993.

  3. Furukawa, T., Kubota, T., Watanabe, M., Kitajima, M., and Hoffman, R.M.  A novel "patient-like" treatment model of human pancreatic cancer constructed using orthotopic transplantation of histologically intact human tumor tissue in nude mice.  Cancer Res. 53, 3070-3072, 1993.

  4. Kubota, T., Inoue, S., Furukawa, T., Ishibiki, K., Kitajima, M., Kawamura, E., and Hoffman, R.M.  Similarity of Serum - Tumor Pharmacokinetics of Antitumor Agents in Man and Nude Mice.  Anticancer Res. 13, 1481-1484, 1993.

  5. Wang, X., Fu, X., Brown, P.D., Crimmin, M.J., and Hoffman, R.M.  Matrix metalloproteinase inhibitor BB-94 (Batimastat) inhibits human colon tumor growth and spread in a patient-like orthotopic model in nude mice.  Cancer Res. 54, 4726-4728, 1994.

  6. Murray, G., Duncan, M., O’Neil, P., Melvin, W., Fothergill, J.  Matrix metalloproteinase-1 is associated with poor prognosis in colorectal cancer.  Nature Medicine 2, 461-2, 1996.

  7. An, Z., Wang, X., Astoul, P., Danays, T., Moossa, A.R., and Hoffman, R.M.  Interferon gamma is highly effective against orthotopically-implanted human pleural adenocarcinoma in nude mice.  Anticancer Res. 16, 2545-2551, 1996.

  8. Olbina, G., Cieslak, D., Ruzdijic, S., Esler, C., An, Z., Wang, X., Hoffman, R., Seifert, W., and Pietrzkowski, Z. Reversible inhibition of IL-8 receptor B mRNA expression and proliferation in non-small cell lung cancer by antisense oligonucleotides.  Anticancer Res. 16, 3525-3530, 1996.

  9. An, Z., Wang, X., Willmott, N., Chander, S.K., Tickle, S., Docherty, A.J., Mountain, A., Millican, A.T., Morphy, R., Porter, J.R., Epemolu, R.O., Kubota, T., Moossa, A.R., and Hoffman, R.M.  Conversion of highly malignant colon cancer from an aggressive to a controlled disease by oral administration of a metalloproteinase inhibitor.  Clinical & Experimental Metastasis 15, 184-195, 1997.

  10. Inada, T., Ichikawa, A., Kubota, T., Ogata, Y., Moossa, A.R., and Hoffman, R.M.  5-FU-induced apoptosis correlates with efficacy against human gastric and colon cancer xenografts in nude mice.  Anticancer Res. 17, 1965-1971, 1997.

  11. Tomikawa, M., Kubota, T., Matsuzaki, S.W., Takahasi, S., Kitajima, M., Moossa, A.R., and Hoffman, R.M.  Mitomycin C and cisplatin increase survival in a human pancreatic cancer metastatic model.  Anticancer Res. 17, 3623-3626, 1997.

  12. Chang, S-G., Kim, J.I., Jung, J-C., Rho, Y-S., Lee, K-T., An, Z., Wang, X., and Hoffman, R.M. Antimetastatic activity of the new platinum analog {Pt(cis-dach)(DPPE)×2NO3} in a metastatic model of human bladder cancer. Anticancer Res. 17, 3239-3242, 1997.

  13. Tomikawa, M., Kubota, T., Matsuzaki, S.W., Takahasi, S., Kitajima, M., Moossa, A.R., and Hoffman, R.M.  Mitomycin C and cisplatin increase survival in a human pancreatic cancer metastatic model.  Anticancer Res. 17, 3623-3626, 1997.

  14. Sasson, A.R., Gamagami, R., An, Z., Wang, X., Moossa, A.R., and Hoffman, R.M.  Cimetidine: an inhibitor or promoter of tumor growth?  International Journal of Cancer 81, 835-838, 1999.

  15. Rho, Y-S., Lee, K-T., Jung, J-C, Yoon, C., An, Z., Hoffman, R.M., and Chang, S-G.  Efficacy of new platinum analog DPPE in an orthotopic nude mouse model of human colon cancer.  Anticancer Res. 19, 157-162, 1999.

  16. Woessner, R., An, Z., Li, X-M., Hoffman, R.M., Dix, R., Bitonti, A. Comparison of three approaches to doxorubicin therapy: free doxorubicin, liposomal doxorubicin, and b-glucuronidase-activated prodrug (HMR 1826).  Anticancer Res. 20, 2289-2296, 2000.

  17. Rashidi, B., An, Z., Sun, F-X., Moossa, A.R., and Hoffman, R.M.  Antimetastatic intraoperative chemotherapy of human colon tumors in the livers of nude mice.  Clinical Cancer Res. 6, 2464-2468, 2000. [PDF] 

  18. Rashidi, B., An, Z., Sun, F-X, Li, X-M., Tang, Z.Y., Moossa, A.R., and Hoffman, R.M.  Efficacy of intra-hepatectomy 5-FU on recurrence and metastasis of human hepatocellular carcinoma in nude mice.  Int. J. Cancer 91, 231-235, 2001.[PDF]

  19. Lee, N.C., Bouvet, M., Nardin, S., Jiang, P., Baranov, E., Rashidi, B., Yang, M., Wang, X., Moossa, A.R., and Hoffman, R.M.  Antimetastatic efficacy of adjuvant gemcitabine in a pancreatic cancer orthotopic model.  Clinical & Experimental Metastasis 18, 379-384, 2001.[PDF] 

  20. Ireson, C.R., Chander, S.K., Purohit, A., Perera, S., Newman, S.P., Parish, D., Leese, M.P., Smith, A.C., Potter, B.V. and Reed, M.J. Pharmacokinetics and efficacy of 2-methoxyoestradiol and 2-methoxyoestradiol-bis-sulphamate in vivo rodents. Br. J. Cancer 90, 932-937, 2004.

  21. Wang, J-W., Yang, M., Yagi, S., and Hoffman, R.M. Oral 5-FU is a more effective antimetastatic agent than UFT. Anticancer Research 24, 1353-1360, 2004. 

  22. Katz MH, Bouvet M, Takimoto S, Spivack D, Moossa AR, Hoffman RM. Survival efficacy of adjuvant cytosine-analogue CS-682 in a fluorescent orthotopic model of human pancreatic cancer.  Cancer Res. 64, 1828-1833, 2004.

  23. Lukashev M, LePage D, Wilson C,  Bailly V,  Garber E, Lukashin A, Ngam-ek A, Zeng W, Allaire N, Perrin S, Xu X, Szeliga K, Wortham K, Kelly R, Bottiglio C, Ding J, Griffith L, Heaney G, Silverio E, Yang W, Jarpe M, Fawell S, Reff M, Carmillo A, Miatkowski K, Amatucci J,  Crowell T, Prentice H, Meier W, Violette SM, Mackay F, Yang D, Hoffman RM, Browning JL.  Targeting the lymphotoxin-β receptor with agonist antibodies as a potential cancer therapy.  Cancer Res. 66, 9617-9624, 2006.

  24. Ammons, W.S., Wang, J.W., Yang, Z., Tidmarsh, G.F., and Hoffman, R.M.  A novel alkylating agent, glufosfamide, enhances the activity of gemcitabine in vitro and in vivo.  Neoplasia 9, 625-633, 2007.

  25. Duan, J.X., Jiao, H., Kaizerman, J., Stanton, T., Evans, J.W., Lan, L., Lorente, G., Banica, M., Jung, D., Wang, J., Ma, H., Li, X., Yang, Z., Hoffman, R.M., Ammons, W.S., Hart, C.P., and Matteucci, M.  Potent and highly selective hypoxia-activated achiral phosphoramidate mustards as anticancer drugs.  Journal of Medicinal Chemistry 51, 2412-2420, 2008.

  26. Matsuo, Y., Raimondo, M., Woodward, T.A., Wallace, M.B., Gill, K.R., Tong, Z., Burdick, M.D., Yang, Z., Strieter, R.M., Hoffman, R.M., Guha, S.  CXC-chemokine/CXCR2 biological axis promotes angiogenesis in vitro and in vivo in pancreatic cancer.  Int. J. Cancer 125, 1027-1037, 2009.

Reviews 

  1. Hoffman, R.M.  Orthotopic is orthodox: Why are orthotopic-transplant metastatic models different from all other models?  J. Cellular Biochem. 56, 1-3, 1994.

  2. Hoffman, R.M.  Fertile seed and rich soil: Development of patient-like models of human cancer by surgical orthotopic implantation of intact tissue.  Update Series: Comprehensive Textbook of Oncology 3, 1-10, Schimpff, S.C., et al, eds. Williams & Wilkins, Baltimore, 1996.

  3. Hoffman, R.M.  Fertile seed and rich soil: The development of clinically relevant models of human cancer by surgical orthotopic implantation of intact tissue.  In:  Teicher, B., ed.  Anticancer Drug Development Guide: Preclinical Screening, Clinical Trials, and Approval.  Totowa, NJ: Humana Press Inc., 127-144, 1997.

  4. Hoffman, R.M.  Orthotopic metastatic mouse models for anticancer drug discovery and evaluation: a bridge to the clinic. Investigational New Drugs 17, 343-359, 1999.[PDF] 

  5. Hoffman, R.M.  Orthotopic transplant mouse models with green fluorescent protein-expressing cancer cells to visualize metastasis and angiogenesis. Cancer and Metastasis Reviews. 17, 271-277, 1999.[PDF] 

  6. Hoffman, R.M.   Clinically Accurate Orthotopic Mouse Models of Cancer.  In: Brooks, S., and Schumacher, U., eds.  Metastasis Research Protocols. Vol. II. Analysis of Cell Behavior In Vitro and In Vivo.  Totowa, NJ: Humana Press Inc., 251-275, 2001.

  7. Hoffman, R.M.   Green Fluorescent Protein for Metastasis Research.  In: Brooks, S., and Schumacher, U., eds. Metastasis Research Protocols. Vol. II. Analysis of Cell Behavior In Vitro and In Vivo.  Totowa, NJ: Humana Press Inc., 285-298, 2001.

  8. Hoffman, R.M.  GFP-Expressing Metastatic-Cancer Mouse Models.  In:  Teicher, B., ed.  Tumor Models in Cancer Research.  Totowa, NJ: Humana Press Inc., 99-112, 2002.

  9. Hoffman, R.M.  Green fluorescent protein imaging of tumor cells in mice.  Lab Animal 31, 34-41, 2002. metastatic

  10.  Hoffman, R.M.  Watching real-time metastasis in vivo.  Trends in Molecular Medicine, 8(7), 354-355, 2002.

  11. Hoffman, R.M.  Whole-body fluorescence imaging with Green Fluorescence Protein.  In: Hicks, B.W., ed.  Methods in Molecular Biology, Vol. 183: Green Fluorescent Protein: Applications and Protocols.  Totowa, NJ: Humana Press, 135-148, 2002.

  12. Hoffman, R.M.  Green fluorescent protein imaging of tumour growth, metastasis, and angiogenesis in mouse models.  Lancet Oncology 3, 546-556, 2002. [PDF] 

  13. Hoffman, R.M. Orthotopic mouse models of Cancer. In: Anticancer Drug Development Guide Preclinical Screeening, Clinical Trials, and Approval, 2nd Ed., pp. 183-212. Teicher, B., Andrews, P.A., eds. Totowa, NJ: Numana Press, 2004.

  14. Glinsky, G.V., Glinskii, A.B., Stephenson A.J., Hoffman, R.M., Gerald W.L. Gene expression profiling predicts clinical outcome of prostate cancer. J. Clin. Invest. 113, 913-923, 2004 [PDF]

  15. Hoffman, R.M. Orthotopic metastatic (MetaMouse® ) models for discovery and development of novel chemotherapy. In: Methods in Molecular Medicine, Vol. III: Chemosensitivity: Vol. II, pp. 297-322, In Vivo Models, Imaging and Molecular Regulators. Blumenthal, R., ed. ISBN #1-58829-586-9. Totowa, NJ: Humana Press, 2005. DOI #10.1226/1588295869.

  16. Hoffman, R.M. Advantages of multi-color fluorescent proteins for whole-body and in in vivo cellular imaging. Journal of Biomedical Optics 10(4), 41202, AN #041202, 2005. [PDF]

  17. Hoffman, R.M.  In vivo cell biology of cancer cells visualized with fluorescent proteins.  In:  “In vivo cellular and molecular imaging” for Current Topics in Developmental Biology, Vol. 70, pp. 121-144. Ahrens, Eric T., ed.  London: Elsevier Life Sciences, 2005.  DOI: 10.1016/S0070-2153(05)70006-5. [PDF]

  18. Mack, G., Marshall, A. Lost in migration. Nature Biotechnology 28, 214-229, 2010 (See page 218, Box 3). Zhang, Q., Yang, M., Shen, J., Gerhold, L.M., Hoffman, R.M., Xing, H.R. The role of the intravascular microenvironment in spontaneous metastasis development. International Journal of Cancer 126, 2534-2541, 2010.  [PDF]

  19. Hoffman, R.  Orthotopic mouse models expressing fluorescent proteins for cancer drug discovery.   Expert Opin Drug Discov. 5, 851-866, 2010.

  20. Hoffman, R.M.  Imaging the steps of metastasis at the macro and cellular level with fluorescent proteins in real time (Chapter 6).  In: Tumor Models in Cancer Research, vol. 2.  Teicher, B.A., ed.  Humana Press (c/o Springer Science+Business Media LLC), 125-166, 2011.

  21. Hoffman, R.M. Imageable clinically-relevant mouse models of cancer. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 141-170. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.

  22. Hoffman, R.M. Imaging metastatic cell trafficking at the cellular level in vivo with fluorescent proteins. In: Methods in Molecular Biology: Metastasis Research Protocols, 2nd ed., 1070, 171-179. Brooks, S.A., Dwek, M., Schumacher, U., eds. Totowa, NJ: Humana Press Inc., 2014.

  23. Hoffman, R.M. The in vivo imaging revolution made by fluorescent proteins. In: The Fluorescent Protein Revolution, Day, R.N., Davidson, M.W., eds. Taylor and Francis Books, 2014, pp 309-324.