Angiogenesis Models - AngioMouse®

LICENSING AND CONTRACT RESEARCH

Features                                                                                                                                    Examples

 

Visualization of tumor vessels using GFP tumor expression

  • Intravital imaging of tumor vessels

  • Whole-body imaging of tumor vessels

  • Quantitation of vessel density

  • Real-time angiogenesis studies

  • Angiogenesis drug discovery and evalation

  • Orthotopic models of all tumor types

 

 

 

Whole-body image of vessels in PC-3-GFP human prostate cancer in nude mouse.

 

Intravital image of ANIP-973-GFP human lung cancer in nude mouse.

 

Whole-body image of Lewis lung-GFP mouse carcinoma in nude mouse.

 

Whole-body images of time course of microvessel development in orthotopic model of MDA-MB-435-GFP human breast cancer.

EXTERNAL IMAGING OF MICROVESSELS OF GFP-EXPRESSING HUMAN PANCREATIC CANCER GROWING ON THE NUDE MOUSE PANCREAS VISUALIZED THROUGH A REVERSIBLE SKIN FLAP.

A - mouse imaged in light box, B - mouse imaged on dissecting microscope.

 

BIBLIOGRAPHY

1.      Yang, M., Li, L., Jiang, P., Moossa, A.R., Penman, S., and Hoffman, R.M.  Dual-color fluorescence imaging distinguishes tumor cells from induced host angiogenic vessels and stromal cells.  Proc. Natl. Acad. Sci. USA 100, 14259-14262, 2003.

2.      Amoh, Y., Li, L., Yang, M., Moossa, A.R., Katsuoka, K., Penman, S., and Hoffman, R.M.  Nascent blood vessels in the skin arise from nestin-expressing hair follicle cells.  Proc. Natl. Acad. Sci. USA 101, 13291-13295, 2004.

3.      Yang, M., Reynoso, J., Jiang, P., Li, L., Moossa, A.R., and Hoffman, R.M.  Transgenic nude mouse with ubiquitous green fluorescent protein expression as a host for human tumors.  Cancer Research 64, 8651-8656, 2004.

4.      Hoffman, R.M.  The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5, 796-806, 2005.

5.      Amoh, Y., Li, L., Yang, M., Jiang, P., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Hair follicle-derived blood vessels vascularize tumors in skin and are inhibited by doxorubicin.  Cancer Res. 65, 2337-2343. 2005.

6.      Amoh, Y., Yang, M., Li, L., Reynoso, J., Bouvet, M., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Nestin-linked green fluorescent protein transgenic nude mouse for imaging human tumor angiogenesis.  Cancer Res. 65, 5352-5357, 2005.

7.      Hoffman, R.M., and Yang, M.  Color-coded fluorescence imaging of tumor-host interactions.  Nature Protocols 1, 928-935, 2006.

8.      Amoh, Y., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., Hoffman, R.M., Bouvet, M.  Dual-color imaging of nascent blood vessels vascularizing pancreatic cancer in an orthotopic model demonstrates antiangiogenesis efficacy of gemcitabine.  J. Surgical Research 132, 164-169, 2006.

9.      Amoh, Y., Nagakura, C., Maitra, A., Moossa, A.R., Katsuoka, K., Hoffman, R.M., and Bouvet, M.  Dual-color imaging of nascent angiogenesis and its inhibition in liver metastases of pancreatic cancer. Anticancer Research 26, 3237-3242, 2006.

10.  Amoh, Y., Bouvet, M., Li, L., Tsuji, K., Moossa, A.R., Katsuoka, K., and Hoffman, R.M.  Visualization of nascent tumor angiogenesis in lung and liver metastasis by differential dual-color fluorescence imaging in nestin-linked-GFP mice.  Clin. Exp. Metastasis 23(7-8), 315-322, 2006.

11.  Amoh, Y., Li, L., Katsuoka, K., Bouvet, M., and Hoffman, R.M.  GFP-expressing vascularization of Gelfoam as a rapid in vivo assay of angiogenesis stimulators and inhibitors.  BioTechniques 42, 294-298, 2007.

12.  Ji, Y., Hayashi, K., Amoh, Y., Tsuji, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Bouvet, M., and Hoffman, R.M.  The camptothecin derivative CPT-11 inhibits angiogenesis in a dual-color imageable orthotopic metastatic nude mouse model of human colon cancer.  Anticancer Res. 27, 713-718, 2007. 

13.  Hayashi, K., Yamauchi, K., Yamamoto, N., Tsuchiya, H., Tomita, K., Amoh, Y., Hoffman, R.M., and Bouvet, M.  Dual-color imaging of angiogenesis and its inhibition in bone and soft tissue sarcoma.  J. Surg. Res., Epub ahead of print, 2007.