A/C Diagnostics Publications

Enzymatic Homocysteine Assay

  1. Stern, P.H., Mecham, J.O. and Hoffman, R.M.   Preparation of [35S]homocysteine thiolactone free of [35S]methionine.  J. Biochemical and Biophysical Methods 7, 83‑88, 1982. [PDF]

  2. Stern, P.H. and Hoffman, R.M. The chemical synthesis of high specific‑activity [35S]adenosylhomocysteine.  Analytical Biochem. 158, 408‑412, 1986. [PDF]

  3. Hoffman, R.M.  Is methioninase useful for the prevention of hyperhomocysteinemia-associated cardiovascular disease? In: Homocysteine Metabolism: From Basic Science to Clinical Medicine, pp. 155-156.  I. Graham, H. Refsum, I.H. Rosenberg, P.M. Ueland, eds.  Massachusetts: Kluwer Academic Publishers, 1997. [PDF]

  4. Han, Q., Lenz, M., Tan, Y., Xu, M., Sun, X., Tan, X-Z., Tan, X-Y., Tang, L., Miljkovic, D., and Hoffman, R.M.  High expression, purification, and properties of recombinant homocysteine a,g-lyase.  Protein Expression & Purification 14, 267-274, 1998. [PDF]

  5. Tan, Y., Tang, L., Sun, X., Zhang, N., Han, Q., Xu, M., Baranov, E., Tan, X-Z., Tan, X-Y., Rashidi, B., An, Z., Perry, A.W., and Hoffman, R.M.  Total-homocysteine enzymatic assay.  Clinical Chemistry 46, 1686-1688, 2000. [PDF]

  6. Tan, Y., Sun, X., Tang, L., Zhang, N., Han, Q., Xu, M., Tan, X., Tan, X., Hoffman, R.M.  Automated enzymatic assay for homocysteine.  Clinical Chemistry 49, 1029-1030, 2003. [PDF]

  7. A/C gets US nod to sell “cheapest” homocysteine test.  Clinica, Issue 1070, August 15, 2003. [PDF]

  8. Han, Q., Sun, X., Xu, M., Zhang, N., Tang, L., Tan, Y., and R.M. Hoffman.  3-deazaadenosine, a stabilizer of whole-blood homocysteine content, does not interfere with the single-enzyme homocysteine assay while totally inhibiting the enzyme conversion homocysteine immunoassay.  Clinical Chemistry 50, 1703-1704, 2004.[PDF]

  9. Tan, Y., and Hoffman, R.M.  A highly sensitive single-enzyme homocysteine assay.  Nature Protocols 3, 1388-1394, 2008. [PDF] Suppl. [PDF]

Vitamin B6 Enzymatic Assay

  1. Han, Q., Xu, M., Tang, L., Tan, X-Z., Tan, X-Y., Tan, Y., and Hoffman, R.M.  Homogeneous nonradioactive enzymatic assay for plasma pyridoxal 5-phosphate.    Clinical Chemistry 48, 1560-1564, 2002. [PDF]

  2. Morris, M.S., Picciano, M.F., Jacques, P.F., Selhub, J. Plasma pyridoxal 5'-phosphate in the US population: the National Health and Nutrition Examination Survey, 2003-2004. Am J Clin Nutr. 87, 1446-1454, 2008. [PDF]

Enzymatic Cysteine Assay

  1. Han, Q., Xu, M., Tang, L., Sun, X., Zhang, N., Tan, X-H., Tan, X-Y., Tan, Y., and Hoffman, R.M.  Homogeneous enzymatic colorimetric assay for total cysteine. Clinical Chemistry 50, 1229-1231, 2004. [PDF]

  2. Han, Q., and Hoffman, R.M. Enzymatic assay for total plasma Cys. Nature Protocols 3, 1778-1781, 2008. [PDF]